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Golgi engineering of CHO cells by targeted integration of glycosyltransferases leads to the expression of novel Asn-linked oligosaccharide structures at secretory glycoproteins

  • Reinl T
  • Grammel N
  • Kandzia S
  • et al.
BMC Proceedings (2013) 7(S6)
DOI: 10.1186/1753-6561-7-s6-p84
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Abstract

We analyze oligosaccharides released from the products of genetically engineered CHO cells based on the resolution of single glycosylation sites of VH- and CH2- glycopeptides by quantitative NP-HPLC-FLD and use our comprehensive oligosaccharide standard library to identify novel oligosaccharide motifs.

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Reinl, T., Grammel, N., Kandzia, S., Grabenhorst, E., & Conradt, H. S. (2013). Golgi engineering of CHO cells by targeted integration of glycosyltransferases leads to the expression of novel Asn-linked oligosaccharide structures at secretory glycoproteins. BMC Proceedings, 7(S6). https://doi.org/10.1186/1753-6561-7-s6-p84

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