Using a handheld gene gun for genetic transformationof tetrahymena thermophila

Abstract

This chapter describes protocols for using a handheld gene gun to deliver transformation vectors for overexpression of genes or gene replacement in the macronucleus of Tetrahymena thermophila. The protocols provide helpful information for preparing Tetrahymena for biolistic bombardment, preparation of vector- coated microcarriers, and basic gene gun operating procedures.