Analysis of 5-Vinyl-l,3-Oxazolidine-2-Thione by Liquid Chromatography

  • Quinsac A
  • Ribaillier D
  • Rollin P
  • et al.
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Abstract

5-Vlnyl-1,3-oxazolldlne-2-thlone (5-VOT) Is a goltrlgenlc compound released by enzymatic degradation of progoltrln, the major glucoslnolate occurring In rapeseed meal. A liquid chromatographic (LC) method for determination of 5-VOT in a biological environment Is presented. Complete extraction of 5-VOT has been carried out by complexatlon with phenyl mercury acetate under cyclohexanlc conditions, and then by decomplexation using an aqueous sodium thlosulfate solution. These reactions displace 5-VOT from an aqueous to an organic medium, and then back again to the aqueous condition, thus assuring high selectivity of the extraction. Precise quantitation of 5-VOT Is completed in 10 mln by reversed-phase liquid chromatography using an isocratic elutlon with UV detection and a specially made synthetic Internal standard. Concentration steps by solid-phase chromatography and evaporation can be introduced In the analytic procedure to lower the detection limit of 5-VOT in the sample used from 100 to 0.5 ppb. Using sow milk samples, the method was tested by small measured additions of 5-VOT. The recovery rate of the product was very good (>97%). Different phases used to achieve a sensitive, rapid, and precise method are described.

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Quinsac, A., Ribaillier, D., Rollin, P., & Dreux, M. (1992). Analysis of 5-Vinyl-l,3-Oxazolidine-2-Thione by Liquid Chromatography. Journal of AOAC INTERNATIONAL, 75(3), 529–536. https://doi.org/10.1093/jaoac/75.3.529

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