Determination of cocaine and metabolites in dried blood spots by LC-MS/MS

Abstract

Cocaine is one of the most common illegal drugs in Europe and other parts of the world. It is mainly metabolized to benzoylecgonine and ecgonine methyl ester but also to minor metabolites such as norcocaine and meta-hydroxy-benzoylecgonine. If ethanol is consumed simultaneously, cocaethylene is formed. Dried blood spots (DBS) are a minimally invasive sampling technique producing easy-to-ship specimens and have garnered increasing attention in forensic and clinical contexts in recent years. We hereby present a liquid chromatography/tandem mass spectrometry-based (LC-MS/MS) method for the quantification of cocaine, benzoylecgonine, ecgonine methyl ester, norcocaine, meta-hydroxy-benzoylecgonine, and cocaethylene in DBS.