Effect of influent ammonia nitrogen concentration on microbial community in MBBR reactor

Abstract

The purpose of this study is to explore the nitrogen removal efficiency of the moving bed biofilm reactor (MBBR) under different ammonia nitrogen concentrations (states P1-P5), especially the composition of various forms of nitrogen, related genes and microbial community structure and succession law in the effluent of the reactor. The results show that the average efficiency of MBBR effluent denitrification is 63.63%. The concentration dynamics of NO3--N and NO2--N in the effluent indicated a relatively short-range nitrification and denitrification reaction in the MBBR. The results of 16SrDNA sequencing of P1-P5 microbial samples found that changes in the concentration of ammonia nitrogen in the influent produced significant changes in the composition of the microbial community in the MBBR. The genera Ottowia and Flavobacterium played an important role in the nitrogen removal of the MBBR system.