Inhibitory effects of oligochitosan on TNF-α, IL-1β and nitric oxide production in lipopolysaccharide.induced RAW264-7 cells

Abstract

Oligochitosan has been reported to possess anti.inflammatory properties; however, the mechanism of the anti-inflammatory effects of oligochitosan remains unknown. The present study aimed to investigate the expression levels of inflammatory cytokines and the production of nitric oxide (NO), in the nuclear factor (NF)κB pathway of lipopolysaccharide (LPS)-stimulated RAW264-7 murine macrophages. The results of the present study demonstrated that different concentrations of oligochitosan could significantly lower the levels of NO, tumor necrosis factor (TNF)-α and interleukin (IL)-1β, released from LPS-stimulated RAW264-7 cells. This was shown to be mediated through inhibiting the activation of the NFκB pathway. These results demonstrate that oligochitosan may efficiently inhibit inflammation and has the potential to be an effective anti.inflammatory agent.