Drosophila pupal (P) wing development entails a series of dynamic developmental events, such as epithelial and glial morphogenesis, that are of outstanding interest to cell biologists. Here, we first describe how to prepare P and prepupal (PP) wings for immunofluorescence microscopy. This protocol has been optimized to visualize wing epithelial architecture, such as polarized cortical domains of planar cell polarity proteins. We then provide a protocol to prepare pupae for whole mount live imaging of P wings. This procedure has allowed us to live-image glial cell migration and proliferation along wing sensory nerves.
CITATION STYLE
Classen, A. K., Aigouy, B., Giangrande, A., & Eaton, S. (2008). Imaging Drosophila pupal wing morphogenesis. Methods in Molecular Biology (Clifton, N.J.), 420, 265–275. https://doi.org/10.1007/978-1-59745-583-1_16
Mendeley helps you to discover research relevant for your work.