Multiplexed Ion Beam Imaging by Time of Flight (MIBI-TOF) enables high-dimensional imaging in situ of clinical specimens at single-cell resolution. In MIBI-TOF, tissue sections are stained with dozens of metal-labeled antibodies, whose abundance and location are read by secondary ionization mass spectrometry. The result is a multi-dimensional image, depicting sub-cellular expression and localization for dozens of distinct proteins in situ. Here, we describe the staining and imaging procedures of a MIBI-TOF experiment.
CITATION STYLE
Elhanani, O., Keren, L., & Angelo, M. (2022). High-Dimensional Tissue Profiling by Multiplexed Ion Beam Imaging. In Methods in Molecular Biology (Vol. 2386, pp. 147–156). Humana Press Inc. https://doi.org/10.1007/978-1-0716-1771-7_10
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