Isolation and Identification of Ssome Lactobacillus Spp. Bacteria and Evaluation Their Efficacy in the Management of Damping off Disease on Peas

Abstract

Twenty-eight isolates of Lactobacillus bacteria were isolated from the rhizosphere of pea plants grown in the fields of five districts in Salah al-Din, which included: Tikrit, Al-Alam, Al-Sharqat, Samarra and Baiji, diagnosed according to phenotypic and biochemical tests. Results showed the effect of L. paralimentarius 1081 on vegetative growth characteristics. Treatment of (bacterial filtrate + Ridomil in the presence of pathogenic fungi) was recording the highest values in dry weight of the vegetative and root systems, was 3.91 and 1.23 g respectively in the local cultivar, compared with the lowest values was 2.60 g and 0.76 g respectively in the Syrian cultivar. All treatments inducing plant resistance compared with healthy plants, and the highest activity of the Peroxidase and Polyphenol oxides in the treatment of (Bacterial filtrate + ridomil in the presence of pathogenic fungi), were 1.08 and 1.33 units/ml in the local cultivar, compared to the Syrian cultivar were 0.015 and 0.013, respectively. Results showed a significant decrease in the severity of infection for all treatments compared to the pathogenic fungus treatment, and the lowest infection severity of the vegetative and root systems was recorded in the treatment of (Bacterial filtrate + ridomil in the presence of pathogenic fungi), which was 14.11 and 12.47% in the local cultivar. There was a significant superiority of all treatments in productivity parameters of pea compared to the treatment of pathogenic fungi only, the highest of those parameters including weight of pods and grains weight/plant were recorded in the treatment (Bacterial filtrate + ridomil in the presence of pathogenic fungi) for the local cultivar was 18.07 g and 14.04 g compared to 10.43 g and 8.20 g in the treatment of the Syrian cultivar with pathogenic fungi only.