Identification of peanut intersectional hybrids with SSR markers

Abstract

Wild peanut relatives constitute an invaluable gene source for broadening the narrow gene base of the cultivated peanut. However, severe pre-/post- fertilization obstacles impeded the utilization of incompatible species within the Arachis genus. In the present study, through SSR profiling, three true intersectional F1 hybrids of Qunyu 101 × Arachis pusilla and a true intersectional F1 hybrid of Huayu 40 × A. pusilla resulting from post-pollination hormone treatment of flower bases were identified, providing strong evidence for the effectiveness of the in vivo hormone treatment method in overcoming cross-incompatibility in the genus Arachis.