Abstract
Candida rugosa lipase was entrapped in silica sol-gel particles prepared by hydrolysis of methyltrimethoxysilane and assayed by p-nitrophenyl palmitate hydrolysis, as a function of pH and temperature, giving pH optima of 7.8 (free enzyme) and 5.0-8.0 (immobilized enzyme). The optimum temperature for the immobilized enzyme (50-55°C) was 19°C higher than for the free enzyme. Thermal, operational, and storage stability were determined with n-butanol and butyric acid, giving at 45°C a half-life 2.7 times greater for the immobilized enzyme; storage time was 21 d at room temperature. For ester synthesis, the optimum temperature was 47°C, and high esterification conversions were obtained under repeated batch cycles (half-life of 138 h). Copyright © 2005 by Humana Press Inc. All rights of any nature whatsoever reserved.
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Soares, C. M. F., De Castro, H. F., Itako, J. E., De Moraes, F. F., & Zanin, G. M. (2005). Characterization of sol-gel bioencapsulates for ester hydrolysis and synthesis. In Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology (Vol. 123, pp. 845–859). Humana Press. https://doi.org/10.1007/978-1-59259-991-2_72
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