Monoclonal antibody detection of a major self peptide. MHC class II complex.

  • Murphy D
  • Rath S
  • Pizzo E
  • et al.
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Abstract

MHC class I and class II molecules transport foreign and self peptides to the cell surface and present them to T lymphocytes. Detection of these peptide:MHC complexes has thus far been limited to analysis of the response of a T cell. Previously, we showed that a mAb, Y-Ae, reacts with 10 to 15% of class II molecules on peripheral B lymphocytes and on cells in the thymus medulla but not thymus cortex in mice that express both I-Ab and I-Eb molecules. Elsewhere, we show that Y-Ae detects a self E alpha peptide bound to I-Ab molecules. Data presented here suggest that the antibody binds over the peptide binding groove of class II molecules, and, like a TCR, appears to recognize both the self peptide and polymorphic class II residues. In addition to B lymphocytes, the Y-Ae determinant is expressed at comparable levels on other APC, including macrophages and dendritic cells. Finally, the antibody does not react with invariant chain-associated class II complexes, thus providing direct evidence that invariant chain:class II complexes and peptide:class II complexes are mutually exclusive. These data provide further evidence that immunologic self is of limited complexity, and have important implications for T cell selection, self tolerance, and autoreactivity.

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Murphy, D. B., Rath, S., Pizzo, E., Rudensky, A. Y., George, A., Larson, J. K., & Janeway, C. A. (1992). Monoclonal antibody detection of a major self peptide. MHC class II complex. The Journal of Immunology, 148(11), 3483–3491. https://doi.org/10.4049/jimmunol.148.11.3483

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