Effect of Vitamins or Lipids Addition on Adenovirus Production at High Cell Densities

Abstract

Methodologies for production of concentrated adenovirus vectors (AdV) with warranted purity and efficacy at low cost are needed as the market for AdV is expanding fast. However, production of AdV that maintain a high specific yield is limited to cell densities in the range of 1×10 6 cell/ml; the nature of the factors for this remains unknown. The aim of this work is to study the effect of addition of vitamins or lipids at the time of infection at high cell densities in order to see if these nutrients are limiting. No improvement on AdV productivity was observed. Furthermore, a significant decrease on cell specific productivity after vitamin addition was observed.