Assessing Polymorphism Information Content (PIC) Using SSR Molecular Markers on Local Species of Citrullus Colocynthis. Case Study: Iran, Sistan-Balouchestan Province

Abstract

Studying polymorphism information content in plants, particularly local plants which are rich in genetic variety, can play an efficient role in building genetic bank and species’ breeding. Iranian colocynth is highly important in terms of medicinal and therapeutic traits and hence, it needs assessing the polymorphism information content. In present research, simple sequence repeat (SSR) markers are used. 32 samples are randomly collected from local accumulations in 8 regions of Sistan-Balouchestan province. DNA extraction from each sample was done using Cetyltrimethylammonium Bromide. 10 primers were designed and used in this study. Polymerase chain reaction was done using extracted DNA and ten primers. To analyze genetic data, NTSYS pc ver.2/2, Genalex 6.5 and XLSATAT software were used. Regarding data analysis of respective values and taking this fact into account that7 out of 10 used primers showed polymorphism information content in respective samples, their classification was not possible and hence, categorization of similar shapes was not performed due to highly genetic diversity of this species and, also, each value had a different shape.