The decline of serum testosterone levels in community-dwelling men over 70 years of age: Descriptive data and predictors of longitudinal changes

Abstract

Objective: This study was designed to assess longitudinal changes in serum testosterone levels, explore relationships with aging, genetic-, health-, and lifestyle-related factors, and investigate predictors of changes in healthy elderly men. Design: Population-based, longitudinal, 4-year observational study in 221 community-dwelling men aged 71-86 years at baseline. Methods: Hormone levels assessed by immunoassay, anthropometry, questionnaires on general health, and genetic polymorphisms. Predictors of changes in testosterone levels explored using linear mixedeffects modeling for longitudinal analyses. Results: Total testosterone (TT), free testosterone, and bioavailable testosterone (BioT) levels decreased with aging, decreases in BioT being most marked. No changes in sex hormone-binding globulin (SHBG) or estradiol (E2 while LH and FSH levels increased during follow-up. Subjects who gained weight displayed a greater decline in TT levels, mainly due to decreasing SUBG levels. However, baseline body composition was not predictive of subsequent changes in testosterone levels. Baseline E2 (P=0.023 to 0.004), LH (P=0.046 to 0.005), and FSH (P<0.002) levels were independently positively associated with a faster decline in testosterone fractions, although only FSH remained significant when adjusting for baseline testosterone (P=0.041-0.035). Carriers of a 'TA' haplotype of the estrogen receptor a gene (ERα) PvuII and Xbal polymorphisms displayed a slower decline of TT and BioT (P=0.041-0.007). Conclusions: In elderly men with already low serum testosterone levels, a further decline was observed, independent of baseline age. The identification of FSH levels as a predictor of this decline appears to reflect the testicular mechanisms of aging-related changes in testosterone production, whereas associations with E2 and ERα polymorphisms are suggestive of estrogen-related processes, possibly related to changes in the neuroendocrine regulation of testosterone production. © 2008 European Society of Endocrinology.