Abstract
The analysis of nuclear envelope components and their function has recently been progressed by the use of computational methods of analysis. The methods in this chapter provided by members of the International Plant Nucleus Consortium address the identification of novel nuclear envelope proteins and the study of structure and mobility of the nucleus. DORY2 is an upgrade of the KASH-finder DORY, and NucleusJ is used to characterize the three-dimensional structure of the nucleus in light microscope images. Finally, a method is provided for analysis of the migration of the nucleus, a key technique for exploring the function of plant nuclear proteins.
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Poulet, A., Zhou, X., Tamura, K., Meier, I., Tatout, C., Graumann, K., & Evans, D. E. (2018). Computational Methods for Studying the Plant Nucleus. In Methods in Molecular Biology (Vol. 1840, pp. 205–219). Humana Press Inc. https://doi.org/10.1007/978-1-4939-8691-0_15
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