Drug development in cell culture: Crosstalk from the industrial prospects

Abstract

The molecular medicine and technology undoubtedly can accelerate the new pharmacophores industrial development. Even though there are many highly-efficient screening methods can be applied in the research, but we still have problems on how to explain these data qualities in the early experimental designs. The researchers recently are just focusing on the animals studies for the drug registration but this method is time-consuming, costly and harmful to animals. Therefore, we need not just focus on the biological parameters and combine them together with the absorption, distribution, metabolism, excretion and toxicity (ADMET) property, permeability and solubility for the optimization study, the cell culture system also play crucial role in the drug candidate development. In conversely to the animals study, the in-vitro cell culture methods are characterized by the low compound requirement and a short duration. Hence, the cell model is suitable for providing us an efficient support for selecting the most promising drug candidate. In this review, we would like to briefly discuss the permeability screening in different stages of drug development, the evaluation on the intestinal permeability of cell culture, the automated Caco-2 cells model determination, the quality control and standard method for the Caco-2 cells determination, the correlation with oral administration absorption fraction and others. © 2014 Lee MKK, et al.