Dynamic Trans Interactions in Yeast Chromosomes

4Citations
Citations of this article
22Readers
Mendeley users who have this article in their library.

Abstract

Three-dimensional organization of the genome is important for regulation of gene expression and maintenance of genomic stability. It also defines, and is defined by, contacts between different chromosomal loci. Interactions between loci positioned on different chromosomes, i.e. "trans" interactions are one type of such contacts. Here, we describe a case of inducible trans interaction in chromosomes of the budding yeast S. cerevisiae. Special DNA sequences, inserted in two ectopic chromosomal loci positioned in trans, pair with one another in an inducible manner. The spatial proximity diagnostic of pairing is observable by both chromosome capture analysis (3C) and epifluorescence microscopy in whole cells. Protein synthesis de novo appears to be required for this process. The three-dimensional organization of the yeast nucleus imposes a constraint on such pairing, presumably by dictating the probability with which the two sequences collide with one another.

Cite

CITATION STYLE

APA

Mirkin, E. V., Chang, F. S., & Kleckner, N. (2013). Dynamic Trans Interactions in Yeast Chromosomes. PLoS ONE, 8(9). https://doi.org/10.1371/journal.pone.0075895

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free