Measurement of oncometabolites D-2-hydroxyglutaric acid and L-2-hydroxyglutaric acid

Abstract

We describe a liquid chromatography-tandem mass spectrometry assay for measurement of d-2-hydroxyglutaric acid and l-2-hydroxyglutaric acid. These metabolites are increased in specific inborn errors of metabolism and are now recognized as oncometabolites. The measurement of d-2-hydroxyglutarate in peripheral blood may be used as a biomarker for screening and follow-up of patients with IDH-mutated acute myeloid leukemia.