Bimolecular fluorescence complementation (BiFC) is a powerful method for studying protein-protein interactions in different cell types and organisms. This method was recently developed in the fruit fly Drosophila melanogaster, allowing analyzing protein interaction properties in a physiologically relevant developing context. Here we present a detailed protocol for performing BiFC with the Venus fluorescent protein in live Drosophila embryos, taking the Hox-PBC partnership as an illustrative test case. This protocol applies to any transcription factor and split fluorescent protein in general.
CITATION STYLE
Duffraisse, M., Hudry, B., & Merabet, S. (2014). Bimolecular fluorescence complementation (BiFC) in live Drosophila embryos. Methods in Molecular Biology, 1196, 307–318. https://doi.org/10.1007/978-1-4939-1242-1_19
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