Protein tyrosine phosphatases (PTP) are a large group of enzymes which work together with protein tyrosine kinases to control the tyrosine phosphorylation of proteins, thus playing a major role in cellular signaling. Here, we provide detailed protocols for expression and purification of the catalytic domain of RPTPµ and full length Eya3 as well as the extracellular region of PTPBR7. Methods are described for evaluation of the purity of the recombinant proteins thus obtained. For the purified Eya3 phosphatase we provide protocols for enzyme activity assay using either chromogenic, fluorescent, or peptide substrates. Determination of kinetic parameters by different graphical and computer-based procedures is also described.
CITATION STYLE
Mentel, M., Badea, R. A., Necula-Petrareanu, G., Mallikarjuna, S. T., Ionescu, A. E., & Szedlacsek, S. E. (2016). Expression, purification, and kinetic analysis of PTP domains. In Methods in Molecular Biology (Vol. 1447, pp. 39–66). Humana Press Inc. https://doi.org/10.1007/978-1-4939-3746-2_3
Mendeley helps you to discover research relevant for your work.