We highly purified O-acetylserine sulfhydrylase from the glutamate-producing bacterium Corynebacterium glutamicum. The molecular mass of the purified enzyme was 34,500 as determined by SDS-polyacrylamide gel electrophoresis, and 70,800 as determined by gel filtration chromatography. It had an apparent Km of 7.0 mM for O-acetylserine and a Vmax of 435 μmol min-1 (mg·protein)-1. This is the first report of the cysteine biosynthetic enzyme of C. glutamicum in purified form.
CITATION STYLE
Wada, M., Awano, N., Yamazawa, H., Takagi, H., & Nakamori, S. (2004). Purification and characterization of O-acetylserine sulfhydrylase of Corynebacterium glutamicum. Bioscience, Biotechnology and Biochemistry, 68(7), 1581–1583. https://doi.org/10.1271/bbb.68.1581
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