Over the last decades, identification of RNA–proteins complexes and their binding sites was challenging. Recently, techniques based on crosslinking, immunoprecipitation, and high-throughput sequencing have been developed. An optimized method, called eCLIP-seq, enables to identify precisely the targeted RNAs as well as the transcriptome-wide binding sites at nucleotide resolution. Here we describe the eCLIP-seq protocol in asexual stages of the human malaria parasite, Plasmodium falciparum. This method could facilitate the characterization of RNA-binding proteins in this organism for which few data are currently available.
CITATION STYLE
Hollin, T., Abel, S., & Le Roch, K. G. (2021). Genome-Wide Analysis of RNA–Protein Interactions in Plasmodium falciparum Using eCLIP-Seq. In Methods in Molecular Biology (Vol. 2369, pp. 139–164). Humana Press Inc. https://doi.org/10.1007/978-1-0716-1681-9_9
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