Microchip electrophoresis, with respect to "profiling of aβ peptides in the cerebrospinal fluid of patients with alzheimer's disease"

Abstract

Aggregation of beta amyloid peptides especially Aβ1-42 in amyloid plaques is one of the major Â? neuropathological events in Alzheimer's disease. This event is normally accompanied by a relative reduction of the concentration of Aβ1-42 in the cerebrospinal fluid (CSF) of patients developing the signs of Alzheimer's disease. Here, we describe a microchip gel electrophoresis method in a polydimethylsiloxane (PDMS) chip that enables rapid profiling of major Aβ peptides. The method was applied to compare the relative concentration of Aβ1-42 with other Aβ peptides, for example, Aβ 1-40 in CSF. In order to increase the sensitivity of detection, Aβ peptides in the CSF samples were first captured and concentrated using magnetic beads coated with specific anti-Aβ antibodies. © 2012 Springer Science+Business Media, LLC.