The taxonomic status of a bacterium, strain NCCP-246 T , isolated from rhizosphere of Vigna mungo, was determined using a polyphasic taxonomic approach. The strain NCCP-246 T can grow at 16-37 C (optimum 32 C), at pH ranges of 6-8 (optimum growth occurs at pH 7) and in 0-4 % (w/v) NaCl. Phylogenetic analysis based upon on 16S rRNA gene sequence comparison revealed that strain NCCP-246 T belonged to genus Sphingobacterium. Strain NCCP-246 T showed highest similarity to the type strain of Sphingobacterium canadense CR11 T (97.67 %) and less than 97 % with other species of the genus. The DNA-DNA relatedness value of strain NCCP-246 T with S. canadense CR11 T and Sphingobacterium thalpophilum JCM 21153 T was 55 and 44.4 %, respectively. The chemotaxonomic data revealed the major menaquinone as MK-7 and dominant cellular fatty acids were summed feature 3 [C 16:1 ω7c/C 16:1 ω6c] (37.07 %), iso-C 15:0 (28.03 %), C 16:0 (11.85 %), C 17:0 cyclo (8.84 %) and C 14:0 (2.42 %). The G+C content of the strain was 39.2 mol%. On the basis of DNA-DNA hybridization, phylogenetic analyses, physiological and, biochemical data, strain NCCP-246 T can be differentiated from the validly named members of genus Sphingobacterium and thus represents as a new species, for which the name, Sphingobacterium pakistanensis sp nov is proposed with the type strain NCCP-246 T (= JCM18974 T = KCTC 23914 T ). © 2013 Springer Science+Business Media Dordrecht.
CITATION STYLE
Ahmed, I., Ehsan, M., Sin, Y., Paek, J., Khalid, N., Hayat, R., & Chang, Y. H. (2014). Sphingobacterium pakistanensis sp nov, a novel plant growth promoting rhizobacteria isolated from rhizosphere of Vigna mungo. Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology, 105(2), 325–333. https://doi.org/10.1007/s10482-013-0077-0
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