Polysome fractionation and analysis of mammalian translatomes on a genome-wide scale

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Abstract

Ribosomes play a central role in protein synthesis. Polyribosome (polysome) fractionation by sucrose density gradient centrifugation allows direct determination of translation efficiencies of individual mRNAs on a genome-wide scale. In addition, this method can be used for biochemical analysis of ribosome- and polysome-associated factors such as chaperones and signaling molecules. © 2014 Creative Commons Attribution-NonCommercial License.

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Gandin, V., Sikström, K., Alain, T., Morita, M., McLaughlan, S., Larsson, O., & Topisirovic, I. (2014). Polysome fractionation and analysis of mammalian translatomes on a genome-wide scale. Journal of Visualized Experiments, (87). https://doi.org/10.3791/51455

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