Polysome fractionation and analysis of mammalian translatomes on a genome-wide scale

Abstract

Ribosomes play a central role in protein synthesis. Polyribosome (polysome) fractionation by sucrose density gradient centrifugation allows direct determination of translation efficiencies of individual mRNAs on a genome-wide scale. In addition, this method can be used for biochemical analysis of ribosome- and polysome-associated factors such as chaperones and signaling molecules. © 2014 Creative Commons Attribution-NonCommercial License.