DNaseI-hypersensitive sites within chromatin are indicative of genomic loci with regulatory function. Several techniques have been described for analyzing these regions, but are either laborious, offer low-throughput possibilities, or are expensive. We have developed a new approach based on a modified version of multiplex ligation-dependent probe amplification (MLPA). Using this method, it is possible to analyse up to 50 defined genomic regions for DNaseI- hypersensitivity in a single PCR-based reaction. This chapter outlines the approach and discusses the critical features of each step of the procedure. © 2012 Springer Science+Business Media, LLC.
CITATION STYLE
Ohnesorg, T., Eggers, S., & White, S. J. (2012). Detecting DNaseI-hypersensitivity sites with MLPA. Methods in Molecular Biology, 786, 201–210. https://doi.org/10.1007/978-1-61779-292-2_12
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