The modification of murashige and skoog media for efficient cultivation of gizella-5 and vsl-2 rootstocks in vitro

Abstract

This paper reports the effect of modified nutrient media on biotechnologically growing rootstock plants (Gizella - 5 and VSL - 2) at the stages of introduction and grafting. Murashige and Skoog medium was modified by adding 25 mg/l Chelate-Fe and 500 mg/l of Ca(NO)3 to the basic nutrient at the stage of introduction of the plant. At the grafting stage 0.1 mg of 6-Benzylaminopurine (6-BAP) was added to Murashige and Skoog, where the effect of regeneration was 2 times higher than in experimental control. The results obtained prove that modified Murashige and Skoog medium is to be the best nutrient media for root-plant introduction and regeneration processes in Vitro followed by Chelate-Fe, Ca(NO)3 and 6-BAP respectively.