Mutagenicity and genotoxicity in gill erythrocyte cells of poecilia reticulata exposed to a glyphosate formulation

Abstract

Poecilia reticulata were exposed to herbicide Roundup Transorb ® for micronucleus test, nuclear abnormalities and comet assay. The exposure-concentrations were based on CL50-96 h following 0, 1.41, 2.83, 4.24 and 5.65 μL L-1 for 24 h. Micronucleus and comets were significantly increased in the gill erythrocyte cells after herbicide exposure compared with the non-exposed group. Results showed a gradual increase in the number of damaged cells, indicating a concentration-dependent effect and that this herbicide was mutagenic and genotoxic to P. reticulata and this effect could be attributed to a combination of compounds contained in the formulation with the active ingredient glyphosate. © 2013 Springer Science+Business Media New York.