Dynamic processes and structural changes of biological molecules are essential to life. While conventional atomic force microscopy (AFM) is able to visualize molecules and supramolecular assemblies at sub-nanometer resolution, it cannot capture dynamics because of its low imaging rate. The introduction of high-speed atomic force microscopy (HS-AFM) solved this problem by providing a large increase in imaging velocity. Using HS-AFM, one is able to visualize dynamic molecular events with high spatiotemporal resolution under near-to physiological conditions. This approach opened new windows as finally dynamics of biomolecules at sub-nanometer resolution could be studied. Here we describe the working principles and an operation protocol for HS-AFM imaging and characterization of biological samples in liquid.
CITATION STYLE
van Ewijk, C., Maity, S., & Roos, W. H. (2024). Visualizing Molecular Dynamics by High-Speed Atomic Force Microscopy. In Methods in Molecular Biology (Vol. 2694, pp. 355–372). Humana Press Inc. https://doi.org/10.1007/978-1-0716-3377-9_17
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