Kinetochore-localized BUB-1/BUB-3 complex promotes anaphase onset in C. elegans

30Citations
Citations of this article
71Readers
Mendeley users who have this article in their library.

Abstract

The conserved Bub1/Bub3 complex is recruited to the kinetochore region of mitotic chromosomes, where it initiates spindle checkpoint signaling and promotes chromosome alignment. Here we show that, in contrast to the expectation for a checkpoint pathway component, the BUB-1/BUB-3 complex promotes timely anaphase onset in Caenorhabditis elegans embryos. This activity of BUB-1/BUB-3 was independent of spindle checkpoint signaling but required kinetochore localization. BUB-1/BUB-3 inhibition equivalently delayed separase activation and other events occurring during mitotic exit. The anaphase promotion function required BUB-1's kinase domain, but not its kinase activity, and this function was independent of the role of BUB-1/BUB-3 in chromosome alignment. These results reveal an unexpected role for the BUB-1/BUB-3 complex in promoting anaphase onset that is distinct from its well-studied functions in checkpoint signaling and chromosome alignment, and suggest a new mechanism contributing to the coordination of the metaphase-toanaphase transition.

References Powered by Scopus

The spindle-assembly checkpoint in space and time

1835Citations
N/AReaders
Get full text

Cyclin synthesis drives the early embryonic cell cycle

1018Citations
N/AReaders
Get full text

S. cerevisiae genes required for cell cycle arrest in response to loss of microtubule function

952Citations
N/AReaders
Get full text

Cited by Powered by Scopus

The Molecular Biology of Spindle Assembly Checkpoint Signaling Dynamics

563Citations
N/AReaders
Get full text

Visualizing the complex functions and mechanisms of the anaphase promoting complex/cyclosome (APC/C)

112Citations
N/AReaders
Get full text

The G2-to-M Transition Is Ensured by a Dual Mechanism that Protects Cyclin B from Degradation by Cdc20-Activated APC/C

57Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Kim, T., Moyle, M. W., Lara-Gonzalez, P., De Groot, C., Oegema, K., & Desai, A. (2015). Kinetochore-localized BUB-1/BUB-3 complex promotes anaphase onset in C. elegans. Journal of Cell Biology, 209(4), 507–517. https://doi.org/10.1083/jcb.201412035

Readers over time

‘15‘16‘17‘18‘19‘20‘21‘22‘23‘2407142128

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 30

50%

Researcher 22

37%

Professor / Associate Prof. 7

12%

Lecturer / Post doc 1

2%

Readers' Discipline

Tooltip

Agricultural and Biological Sciences 31

49%

Biochemistry, Genetics and Molecular Bi... 30

48%

Social Sciences 1

2%

Chemistry 1

2%

Save time finding and organizing research with Mendeley

Sign up for free
0