Synthesis and enzymatic stability of oligonucleotides consisting of isonucleosides

Abstract

Novel oligodeoxynucleotide analogues consisting of deoxyisonucleosides were obtained by the phosphoramidite approach on automated DNA-synthesizer. The phosphoramidite building blocks were synthesized by phosphorylation of corresponding isonucleosides. The oligodeoxynucleotide analogues A, B and C were evaluated with respect to hybridization properties and enzymatic stabilities. The oligomers are all stable towards snake venom phosphodiesterase, but only oligomer B and C displayed acceptable hybridization properties to complementary dA14. © 1999 IUPAC.