The locations of DNA binding by the cyclic AMP receptor protein (CRP) in the deo operon of Escherichia coli have been determined by the DNase I footprinting procedure. Two high affinity sites were found around positions -35 and -90, preceding the second deo promoter. In vitro data on induction of gene fusions that join different parts of the deoP -2 regulatory region to the lac genes suggest that: (1) both CRP binding sites are needed for high expression from the deoP -2 region; and (2) negative regulation by the cytR repressor is accomplished by preventing the cAMP-CRP complex from binding to the second target.
CITATION STYLE
Valentin-Hansen, P. (1982). Tandem CRP binding sites in the deo operon of Escherichia coli K-12. The EMBO Journal, 1(9), 1049–1054. https://doi.org/10.1002/j.1460-2075.1982.tb01295.x
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