Tandem CRP binding sites in the deo operon of Escherichia coli K-12.

43Citations
Citations of this article
7Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

The locations of DNA binding by the cyclic AMP receptor protein (CRP) in the deo operon of Escherichia coli have been determined by the DNase I footprinting procedure. Two high affinity sites were found around positions -35 and -90, preceding the second deo promoter. In vitro data on induction of gene fusions that join different parts of the deoP -2 regulatory region to the lac genes suggest that: (1) both CRP binding sites are needed for high expression from the deoP -2 region; and (2) negative regulation by the cytR repressor is accomplished by preventing the cAMP-CRP complex from binding to the second target.

Cite

CITATION STYLE

APA

Valentin-Hansen, P. (1982). Tandem CRP binding sites in the deo operon of Escherichia coli K-12. The EMBO Journal, 1(9), 1049–1054. https://doi.org/10.1002/j.1460-2075.1982.tb01295.x

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free