We have built a series of Neurospora crassa strains containing alleles of green fluorescent protein (GFP) to provide a visual phenotype for investigating meiotic recombination. These strains provide a convenient means of screening the Neurospora knockout library for genes involved in genetic recombination. They permit rapid analysis of recombination outcomes by allowing visualization of segregation patterns in a large number of octads from crosses heterozygous for GFP. Using this system the effect of a knockout on gene conversion and/or on crossing over between the fluorescent marker and the centromere can be measured.
CITATION STYLE
Bowring, F. J., Yeadon, P. J., & Catcheside, D. E. A. (2017). Fluorescent protein as a tool for investigating meiotic recombination in Neurospora. In Methods in Molecular Biology (Vol. 1471, pp. 133–145). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6340-9_6
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