Density-dependent Growth Patterns of Meloidogyne javanica on Hemp Cultivars: Establishing Nematode-sampling Timeframes in Host-status Trials

Abstract

The timeframe host plants should be exposed to Meloidogyne species prior to nematode sampling for host-status and/or host-sensitivity is indispensable in interpretation of results and the subsequent management decisions. The aim of this study was to use density-dependent growth patterns of population density of M. javanica infecting hemp (Cannabis sativa) cultivars to determine the timeframe from inoculation to nematode-sampling in host-status trials. Seedlings of four hemp cultivars 'Kompolti', 'Futura-75', 'Ferimon' and 'Felina-34' were each inoculated with 1000 second-stage juveniles of M. javanica under greenhouse conditions, with four cultivars (main-plots) and five biweekly nematode-sampling times (sub-plots) arranged in a split-plot design, with five replications (n=100). Interaction and cultivar type had no significant (P=.05) effects on population density of M. javanica, while increasing sampling time had highly significant (P