Antimicrobial photodynamic of blue LED for activation of curcumin extract (curcuma longa) on staphylococcus aureus bacteria, an in vitro study

Abstract

Staphylococcus aureus (S.aureus) is a normal skin flora. In abnormal conditions, these bacteria can cause mild skin infections such as acne vulgaris and cellulitis folliculitis that attack the respiratory system causing severe infections such as pneumonia and empyema occur. Systemic treatment with antibiotics for a long time can cause resistance. Antimicrobial photodynamic therapy (aPDT) is a therapy that uses light sources, photosensitizer (PS) agents and oxygen. This study aims to determine the effectiveness of blue LED (400-450) nm to activate exogenous PS of curcumin extract in S.aureus in vitro. To determine the antibacterial effect of curcumin extract (Curcuma longa) on S.aureus bacteria with LED radiation, the distribution of samples was divided into four treatment groups, Group (S - K -) control group without any treatment, group (S- K +) control group with curcumin extract, group (S + K-) group with LED irradiation without curcumin extract, group (S + K +) laser irradiation group with curcumin extract. Bacterial growth is measured using Elisa Reader and Total Plate Count Method. The results showed that the LED energy density 16.19 J/cm2 was able to increase the percentage of bacterial mortality by (91.49 ± 0.01) % with curcumin extract and (44.88 ± 0.18) % without curcumin extract. So LED irradiation is able to activate curcumin extract to increase the percentage of S.aureus bacterial death.