Multiple isotope tracers in fur keratin discriminate between mothers and offspring

Abstract

Rationale: Isoscape origin models for mammals may be impaired by fractionation and routing of isotopes during lactation. Here, we tested if the stable carbon, nitrogen and hydrogen ratios of juvenile bats differ from those of their mothers and if derived isotopic dietary niches and geographical assignments vary accordingly between mothers and juveniles. Methods: During the post-lactation period, we collected fur of juvenile and female common noctule bats (Nyctalus noctula) from the same maternity roost. Using a combination of elemental analysis and stable isotope ratio mass spectrometry, we measured the hydrogen, carbon and nitrogen isotope ratios in fur keratin. The hydrogen isotope ratios were measured for the non-exchangeable portion of hydrogen in keratin. The derived isotopic niches and isoscape origin models were compared between mothers and juveniles. Results: The fur keratin of juveniles was enriched by 1.6‰ in 15N and depleted by 2.9‰ in 13C compared with that of the mothers. In addition, the hydrogen isotope ratios were 13.4‰ lower in the fur keratin of juveniles than in that of mothers. The estimated isotopic niches of nursing females and juveniles were not overlapping and differed in size. Overall, the isoscape origin models projected juveniles as being from a more northern origin than the mothers; yet both models suggested the study site as a likely place of origin. Conclusions: Our results suggest that isotope ratio data of juvenile bats should not be used for transfer functions in isoscape origin models because of isotopic routing and discrimination during lactation. Not accounting for age effects may increase the inaccuracy of geographical assignments in mammals when based on stable hydrogen isotopes.