The Xenopus oocyte is a widely used system for protein expression. Investigators have had the choice between two different techniques: injection into the cytoplasm of in vitro transcribed complementary RNA (cRNA) or injection into the nucleus of complementary DNA (cDNA). We report on a third expression technique that is based on the combined injection of cDNA and purified T7 RNA polymerase directly into the cytoplasm of oocytes.
CITATION STYLE
Altafaj, X., Joux, N., Ronjat, M., & De Waard, M. (2006). Oocyte expression with injection of purified T7 RNA polymerase. Methods in Molecular Biology (Clifton, N.J.). https://doi.org/10.1007/978-1-59745-000-3_5
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