The effect of zebularine on the heat-activated retrotransposon onsen in arabidopsis Thaliana and Vigna angularis

Abstract

The Ty1/copia-like retrotransposon ONSEN is conserved among Brassica spe-cies, as well as in beans, including adzuki bean (Vigna angularis (Willd.) Ohwi & Ohashi), which is one of the economically important crops in Japan. ONSEN has acquired a heat-responsive element that is recognized by plant heat stress defense factors, resulting in its transcription and the production of full-length extrachromo-somal DNA under conditions with elevated temperatures. DNA methylation plays an important role in regulating the activation of this transposon in plants. There-fore, chemical inhibition of DNA methyltransferases has been utilized to study the effect of DNA methylation on transposon activation. To understand the effect of DNA methylation on ONSEN activation, Arabidopsis thaliana and adzuki bean seedlings were treated with zebularine, which is known to be an effective chemical demethylation agent. The results showed that ONSEN transcription levels were upregulated in zebularine-treated plants. Extrachromosomal DNA of ONSEN also accumulated in the treated plants.