An unmet need exists for a clinical diagnostic to determine preterm birth (PTB) risk. Such an assessment is possible with high sensitivity and specificity using a panel of nine biomarkers. An integrated microfluidic analysis system for these biomarkers is being developed which includes microchip electrophoresis (μCE) separation. A t-shaped microchip device can be used to test the μCE portion of this integrated system to find appropriate separation conditions. These t-shaped microchips can be fabricated using photolithographically patterned Si templates and hot embossing. PTB biomarkers can be fluorescently labeled using an amine-reactive dye prior to μCE. The μCE conditions established using this t-shaped device should be useful in developing a complete integrated microfluidic system for PTB risk assessment.
CITATION STYLE
Nielsen, A. V., & Woolley, A. T. (2019). Device fabrication and fluorescent labeling of preterm birth biomarkers for microchip electrophoresis. In Methods in Molecular Biology (Vol. 1972, pp. 175–184). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9213-3_12
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