Biological Control of Fusarium oxysporum in Tomato Seedling Production with Mexican Strains of Trichoderma

Abstract

The problems and limitations of the control of diseases caused by phytopathogens through the use of fungicides, make the biological control present as an alternative method in the production of tomato plants in greenhouse, which is limited by the incidence of Fusarium oxysporum Schlechtend.:Fr., being the most worldwide destructive disease. The objective of the present investigation was to evaluate the effect of three Mexican strains of the genus Trichoderma against F. oxysporum on the production of tomato seedlings under greenhouse conditions, as well as to determine the antagonistic effect of the strains used. The Trichoderma harzianum strain had the highest antagonistic activity (81.50%) and the highest growth rate (1.25 cm/day), proving to be the most aggressive strain to control F. oxysporum. in addition the results of the interaction of the dual cultures paired, presented a visible overgrowth zone with hyphae of Trichoderma spp. Seeds inoculated with T. harzianum showed a survival of 84% and a mortality of 16%, lower than the control group, which present a mortality of 58%; however, the treatment inoculated with F. oxysporum had the highest incidence of "disease" with 83%, the lowest survival (17%) and a decrease of the green biomass with respect to the control.