The cytotoxic activity on T47D breast cancer cell of genistein-standardized ethanolic extract of tempeh - A fermented product of soybean (Glycine max)

Abstract

As fermented soybean (Glycine max), tempeh has been reported as a good source of isoflavones, which have shown anticancer activities in various cancer cells resulted from their activity as phytoestrogens. In this article, the preparation of ethanolic extract of tempeh is presented and then followed by the exploration of its cytotoxic effect on T47D cells. The extract was subsequently standardized using genistein, the major phytoestrogen found in tempeh. The genistein concentration found in the extract of tempeh was 0.681 % (w/w). The cytotoxicity assay employing 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) resulted in IC50 values of 196.066 ± 15.956 μg/mL and 13.174 ± 0.905 μg/mL for the genistein-standardized ethanolic extract of tempeh and genistein, respectively.