Mesenchymal stem cells (MSCs) are blossoming as a credible source for regenerative medical applications. The use of fetal MSCs is gaining momentum for therapeutic use. The ease of isolation, enhanced characteristics, and immunomodulation properties renders the utilization of fetal MSCs for numerous clinical applications. In this article, we will demonstrate a step-by-step protocol for isolation of Wharton’s jelly MSCs (WJMSCs) from the human umbilical cord matrix, preparation of human platelet lysate, fabricating amniotic membrane scaffold and mice model to study skin regeneration using a combination of MSCs and decellularized amniotic membrane scaffold.
CITATION STYLE
Sabapathy, V., Sundaram, B., & Kumar, S. (2017). Therapeutic application of human Wharton jelly mesenchymal stem cells in skin injury of SCID. In Methods in Molecular Biology (Vol. 1553, pp. 115–132). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6756-8_9
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