The principal challenges facing PCR-based analyses of DNA extracted from formalin-fixed materials are fragmentation of the DNA and cross-linked protein-DNA complexes. Here, we present an efficient protocol to extract DNA from formalin-fixed or paraffin-embedded tissues (FFPE). In this protocol, protein-DNA cross-links are reversed using heat and alkali treatment, yielding significantly longer fragments and larger amounts of PCR-amplifiable DNA than standard DNA extraction protocols. © 2012 Springer Science+Business Media, LLC.
CITATION STYLE
Campos, P. F., & Gilbert, T. M. P. (2012). DNA extraction from formalin-fixed material. Methods in Molecular Biology, 840, 81–85. https://doi.org/10.1007/978-1-61779-516-9_11
Mendeley helps you to discover research relevant for your work.