Evaluation of Antibacterial Activity and GCMS Analysis of Zanthoxylum Ovalifolium Fruit Extracts

Abstract

Herbal medicines have played a vital role in the evolution of humanity and civilization. The fruits of the plant Zanthoxylum were collected from the study field, washed, dried in the shade and powdered. Soxhlation method was used to make different solvent extract of fruit. The different fruit extracts were examined against P. aeruginosa, P. syringe, S. aureus, S. typhi, K. pneumonia and E. coli by agar well diffusion method and for the detection of biochemical components present in the Zanthoxylum fruit, methanolic fruit extract was analyzed using GC-MS. Results showed that, the methanolic extract of fruit is found to be exhibit activity against P. aeruginosa, P. syringe, S. aureus, S. typhi, K. pneumonia and E. coli when compared to the hexane and ethyl acetate. The discovery of 46 bioactive compounds in methanolic fruit extract was verified by their molecular formula, molecular weight, and area peak percentage. In which 6 bioactive compounds such as 6-octadecanoic acid, Pentadecanoic acid, Ethyl oleate, 9-octadecenoic acid (Z)-, methyl ester, Glycerin and 1, 2-dimethoxy-4-(2-methoxyethenyl) benzene compounds showed highest peak area percentage of 26.14, 13.61, 8.56, 6.12 and 6.08% respectively. The compounds found in this research may be responsible for some of the medicinal action of the Z. ovalifolium fruit and may be useful in the development of new medicines.