SULPHATED DERIVATIVES OF LAMINARIN

Abstract

The polysaccharide, laminarin, was prepared from the marine alga, Laminaria digitata, and was sulphated with chlorosulphonic acid in pyridine and in liquid sulphur dioxide at temperatures below 0°. Derivatives containing both O-sulphate and N-sulphate groups were prepared by sulphating β-aminoethyl ethers of laminarin obtained by the reaction of laminarin with ethylenimine. These derivatives were found to act as anticoagulants for blood in vitro. The preparations with highest sulphate were most active, and for equivalent sulphate that with both O-sulphate and N-sulphate groups was more active than the one containing only the former.