Nitric oxide-forming reaction between the iron-N-methyl-D-glucamine dithiocarbamate complex and nitrite

Abstract

The objective of this study was to elucidate the origin of the nitric oxide-forming reactions from nitrite in the presence of the iron-N-methyl-D- glucamine dithiocarbamate complex ((MGD)2Fe2+). The (MGD)2Fe2+ complex is commonly used in electron paramagnetic resonance (EPR) spectroscopic detection of NO both in vivo and in vitro. Although it is widely believed that only NO can react with (MGD)2Fe2+ complex to form the (MGD)2Fe2+·NO complex, a recent article reported that the (MGD)2Fe2+ complex can react not only with NO, but also with nitrite to produce the characteristic triplet EPR signal of (MGD)2Fe2+·NO (Hiramoto, K., Tomiyama, S., and Kikugawa, K. (1997) Free Radical Res. 27, 505-509). However, no detailed reaction mechanisms were given. Alternatively, nitrite is considered to be a spontaneous NO donor, especially at acidic pH values (Samouilov, A., Kuppusamy, P., and Zweier, J. L. (1998) Arch Biochem. Biophys. 357, 1-7). However, its production of nitric oxide at physiological pH is unclear. In this report, we demonstrate that the (MGD)2Fe2+ complex and nitrite reacted to form NO as follows: 1) (MGD)2Fe2·NO complex was produced at pH 7.4; 2) concomitantly, the (MGD)3Fe3+ complex, which is the oxidized form of (MGD)2Fe2+, was formed; 3) the rate of formation of the (MGD)2Fe2+·NO complex was a function of the concentration of [Fe2+]2, [MGD], [H+] and [nitrite].