Morphology of Fresh and Cryopreserved Sperms of Banana Shrimp Penaeus merguiensis (De Man, 1888)

Abstract

The spermatophore morphology of the P. merguiensis from Kedah, Malaysia is described. About 10 cryopreserved groups as 6,12 and 24 h, 7, 30, 60, 90, 120, 150 and 180 days and fresh as control was examined from each of three replication were evaluated for sperm gross morphology evaluations. A fully mature male's broodstock of P. merguiensis was taken with fresh spermatophores and evaluated for sperm morphologically. Cryopreserved spermatophore after the thawing 27 oC/2 min (fresh and frozen) individually transferred into glass homogenizer (High speed variable speed reversible, Glas-col, Terre Havte In USA) with 200 uL of Ca-F saline. Fixation, dehydration by series of alcohol, Critical Point Dry (CPD) and mount specimens on to stubs using or carbon dots as well as using Auto Fine Coater and Sputter Coater moreover scanning by Model JEOL 6360LA scanning electron microscope. The cryopreserved spermatophore shows similarities with those of fresh, there were no significant differences (p>0.05) between the freshly spermatophore and spermatophorestored up to 90 days at -196 oC liquid nitrogen. © Medwell Journals, 2012.