Optimization of medium for exopolysaccharide production by Agaricus brunnescens

Abstract

The exopolysaccharide of Agaricus brunnescens is a newly produced biomaterial for various applications. In this paper, we optimized the culture medium for this exopolysaccharide production by A. brunnescens using orthogonal methods. The optimal medium for enhancing production was determined as wheat bran 120 g/ L, Glucose 25 g/L and peptone 2.0 g/L for exopolysaccharide yield. This optimization strategy in shake flask culture leads to an exopolysaccharide production of 12.13 g/L, which was considerably higher than that obtained in preliminary studies.