Forensic analysis of mitochondrial and autosomal markers using Pyrosequencing®

1Citations
Citations of this article
6Readers
Mendeley users who have this article in their library.
Get full text

Abstract

Forensic casework analyses often face challenges, such as limited genetic material with or without fragmentation and damage. To compensate for low amounts and degradation, shorter amplicons are often applied in the analysis. Also, a change of markers might be necessary using mitochondrial instead of autosomal markers. In addition, forensic research often involves analysis of large number of samples for marker evaluation and population-database compilation. Therefore, a flexible, robust but also rapid method for the detection of variation is highly useful. Pyrosequencing ® is a rapid, reliable, easy-to-use method for sequence analysis. The method is well suited for rapid forensic analysis of a few targets or analysis of a single target in many samples. It allows sequencing of very short amplicons, which facilitates analysis of degraded DNA. Here we present the use of Pyrosequencing, a robust method for sensitive forensic analysis of mitochondrial DNA, autosomal STRs, and Y-chromosome STRs and SNPs.

Cite

CITATION STYLE

APA

Buś, M. M., Edlund, H., & Allen, M. (2015). Forensic analysis of mitochondrial and autosomal markers using Pyrosequencing®. Methods in Molecular Biology, 1315, 379–396. https://doi.org/10.1007/978-1-4939-2715-9_26

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free